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3B). R526 from your loop amongst αJ and αI anchors the C-terminal part of the activation loop by forming a salt bridge with E458 at the base of αEF. Q459 stabilizes the HRD motif by a hydrogen bond to the key chain carbonyl of R413. The tip in get more info the activation segment is stabilized by a hydrogen bond among Y454 and E480 from αfile.

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